عنوان مقاله [English]
Nowadays the uterine infections are considered as one of the principal disorders of bitch’s reproductive system. For
this reason, the utilization of lactobacillus spp. as probiotic and aimed at substituting in the uterus as an inhibiting factor
against the uterine infections due to the production of metabolites such as hydrogen peroxide and lactic acid be useful
and should be investigated (13). For this reason, in order to isolate the lactobacillus spp. from the vaginas of healthy
bitches beings, an attempt was made to obtain specimens using double guard swab under sterile conditions from twenty
healthy bitches which had been primarily confrmed through radiograph, ultrasonography and clinical examinations.
Subsequent to the acquisition of specimens, the swabs were immediately placed in MRS-broth medium and after
transferring to laboratory they were incubated frstly for 48 to 72 hours in anaerobic condition under 37ºc. Thereafter,
from the specimens grown on MRS-agar, cultures were prepared and further incubated under above conditions for
more 48 to72 hours. Utilizing direct observation, biochemical tests and ultimately PCR and sequencing the genus of
lactobacillus spp. was discerned from the colonies grown on the surface. Following this step, the lactobacillus spp.
were subjected to isolation and propagation. On the other hand, in a bid to test the effect of isolated lactobacillus on
regular pathogens occurring in uterine infections, a certain number of afﬂicted bitches beings referred to medical clinics
existing all over Alborz province were selected for obtaining specimens of uterine secretions. In continuation, the degree
of created inhibition zone resulting from separate proximity of lactobacilli and antibiogram discs with main isolated
pathogens including Staphylococcus intermedius, E. coli, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus
aureus and Trueperella were compared. The fndings demonstrated that the Lactobacilli had a signifcant effect on
a multitude of pathogenic bacteria for the creation of inhibition zone from growth in laboratory conditions. Most of
isolated pathogens (83.3%) in the vicinity of 2.5µl of fresh culture medium of Lactobacillus showed susceptibility which
indicates a signifcant difference in comparison with susceptibility of all antibiogram discs under investigation (34.16%).
In the light of acquired results in laboratory conditions (in vitro), it seems that the application of Lactobacillus spp. in bitch
vagina as a preventive factor from uterine infections or as a factor for mitigating the disposition to such infections should
also be surveyed under clinical conditions which might possibly be a suitable replacement for antibiotic treatments.