نویسندگان
1 دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، دانشکده علوم تخصصی دامپزشکی، گروه پاتوبیولوژی، تهران، ایران
2 دانشگاه تهران، دانشکده دامپزشکی، گروه بهداشت و کنترل مواد غذایی، تهران، ایران
3 دانشگاه علوم پزشکی تهران، دانشکده بهداشت، گروه اپیدمیولوژی و آمار زیستی، تهران، ایران
4 دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، دانشکده علوم تخصصی دامپزشکی، گروه بهداشت، تهران، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Somatic cell count (SCC) has been considered as the golden standard method to evaluate raw bulk milk quality for
several decades. Nevertheless, SCC is neither sensitive nor specifc enough to evaluate raw bulk milk. Much effort
has been performed to substitute new markers in this regard. Acute phase proteins (APPs) as part of inflammatory
response received too much attention as new diagnostic markers in veterinary medicine as well as subclinical
mastitis in dairy cattle. Since subclinical mastitis cause unfavorable changes in milk composition, APPs would
be a potential biomarkers to predict milk quality. The objectives of this study were to determine the diagnostic
value of Milk Amyloid A(MAA) for evaluating raw bulk milk quality delivered to dairy industry and to establish
cut off points to detect early changes in bulk tank milk quality. The study performed on 30 random selected dairy
farms delivering their bulk milk to Pegah dairy industries at Tehran province. Three milk samples collected at
3 different times to measure MAA, SCC, total protein, casein, total fat and lactose level using commercially
available methods. Diagnostic sensitivity and specifcity and cutoff points for each test were determined using
receiver-operating characteristics (ROC) analysis. MAA was the most accurate test with a diagnostic sensitivity
of 96% and specifcity of 60% at cutoff point of 55.64ng/mL based on a SCC of 200 × 1000 cells/mL of bulk
milk. The study showed that determination of MAA can be used as a potential biomarker to discriminate early
unfavorable compositional change in bulk milk quality based on SCC higher than 200 × 1000 cells/mL of bulk
milk. A signifcant relationship found between MAA and bulk milk protein quality traits(P<0.05).
کلیدواژهها [English]