عنوان مقاله [English]
Dicrocoelium and Fasciola are hepatic duct parasites of different animals that are reported from cattle, buffalo,
camel, sheep, goats, wild rabbit and hogs in Iran and many other parts of the world. Studies show that the existence
of this parasites in the livers of final hosts become pathogens.The new technologies in the field of molecular
biology had caused basic changes in the classic researches and indistinguishing parasites. One of these methods is
the somatic proteins identification of the parasites resulted from SDS-PAGE.
In an abattoir, adult trematodes were obtained from infested livers of cows, sheep and goats. The different parts
of the parasite, such as evacuated materials, excretory and somatic parts can have antigens for diagnosing proteins.
There are different method for diagnosing proteins. The best and easiest of these methods is electrophoresis with
SDS PAGE. After some processing, some adult termatodes of every host for the expulsion of extra materials was
washed in PBS (pH= 7.4) 3-4 times and the resulting solution was centrifuged in 4oC for 30 min in 12000 G.
The results of the present study showed that D. denderiticum protein bands are in 14.4-116 kDa range and their
numbers is 15. F. hepatica protein bands are in 18-63 kDa and include 7 bands, while F. gigantica in the same
range is 9 bands.
Using somatic proteins pattern can be considered as a new paraclinic method for the differential diagnosis of
Fascioliasis and Dicrocoeliasis.